羊乳及其制品中掺入牛乳成分的双重PCR检测

被引:11
作者
宋宏新
刘建兰
徐秦峰
机构
[1] 陕西科技大学食品与生物工程学院
关键词
羊乳掺假; 牛乳; DNA提取; PCR检测;
D O I
10.19481/j.cnki.issn2096-398x.2018.01.007
中图分类号
TS252.7 [产品标准与检验];
学科分类号
083203 ;
摘要
根据牛、羊的线粒体12SrRNA基因为靶基因设计合成两对特异性引物,建立了羊乳中掺入牛乳的双重PCR检测方法,对鲜乳中快速DNA提取进行了快速法和磁珠法的比较研究.结果显示,利用快速法提取出的DNA纯度欠佳,羊乳中能检测出牛乳10%的掺入比例,而利用磁珠法提取的DNA纯度高、重复性好,在羊乳及其制品中掺入牛乳检出值能达到1%,将该检测方法应用于巴氏杀菌乳和不同品牌市售羊奶粉的掺假牛乳的检验验证.以磁珠法来提取DNA,双重PCR检测方法可以作为羊乳及其制品中掺入牛乳源性成分的有效手段之一,为羊乳行业的掺假行为提供一定技术依据.
引用
收藏
页码:34 / 38+44 +44
页数:6
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