Immunocytochemical identification of actin in mitochdria of Physarum polycephalum

被引：0

作者：

ZENG Xianlu XING Miao WANG Xiaoguang SONG Zhaoxia and HAO ShuiInstitute of Genetics and Cytology Northeast Normal University Changchun China ^{[130024
]}

Shenzhen University Shenzhen China ^{[518086
]}

Department of Biology Changchun Normal College Changchun China ^{[130032
]}

Fundamental Medical College Jilin University Changchun China ^{[130021
]}

机构：

来源：

ProgressinNaturalScience | 2003年 / 01期

关键词：

Physarum polycephalum; mitochondria; actin;

D O I：

暂无

中图分类号：

O656 [有机分析];

学科分类号：

070302 ; 081704 ;

摘要：

<正> Abstract Mitochondria isolated from the plasmodia of Physarum polycephalum Schw. are reacted with rabbit anti-actin anti-body, and detected with FITC-conjugated sheep anti-rabbit IgG antibody. The results of indirect immunofluorescence show that actin ex-ists in the mitochondria. Western blot analysis confirms the existence of actin in the protein preparation of the mitochondria. The indirectimmunoelectron microscopic observation using the same antibodies verifies further that actin is the constituents of mitochondria, and it isdispersively distributed in the mitochondria of P. polycephalum.

引用

页码：48 / 51

页数：4

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