Construction Strategy and Affecting Factors of Positive-Strand RNA Virus Infectious Clone

被引:0
作者
SHEN Shichuanand YUE Kuizhong College of Life SciencesNortheast Agricultural UniversityHarbin China [150030 ]
机构
关键词
positive-strand RNA virus; infectious clone; affecting factors; application;
D O I
暂无
中图分类号
Q785 [转化及克隆];
学科分类号
071007 ; 0836 ; 090102 ;
摘要
Construction of infectious clones by full-length cDNA is basic and key for recovering RNA virus and is core of reverse genetics.In this article,basic consideration and key technology were viewed and factors affecting infectivity of clones were also summarized.Some research advances were briefly introduced about positive-strand RNA viruses infectious clones.Finally,this article also reviewed the application of infectious clones.
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页码:80 / 87
页数:8
相关论文
共 2 条
[1]  
Development and analysis of a tick-borne encephalitis virus infectious clone using a novel and rapid strategy[J] . T.S. Gritsun,E.A. Gould.Journal of Virological Methods . 1998 (1)
[2]  
Infectious cucumber mosaic virus RNA transcribed in vitro from clones obtained from cDNA amplified using the polymerase chain reaction .2 Hayes R.J,Buck K.W. Journal of General Virology . 1990