Expression of Cystic Fibrosis Transmembrane Conductance Regulator in Rat Ovary

被引：0

作者：

靳镭

汤瑞玲

机构：

[1] DepartmentofObstetricsandGynecology,TongjiHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology

来源：

Journal of Huazhong University of Science and Technology(Medical Sciences) | 2008年 / 05期

关键词：

cystic fibrosis transmembrane conductance regulator; Cl-; channel; rat; ovary; follicle; corpus luteum;

D O I：

暂无

中图分类号：

R711.7 [女性生殖器其他疾病];

学科分类号：

100211 ;

摘要：

The protein expression of cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated Cl- channel, in ovarian stimulated premature female rat ovary during a cycle of follicle development and corpus luteum formation was investigated. Animals were injected with 10 U pregnant Mare's serum gonadotropin (PMSG) and subsequently 10 U hCG 48 h later. Time-dependent immunohistochemistry and Western blotting experiments were performed before and 24, 48, 72 h after hCG treatment. The immunohistochemistry revealed that administration of PMSG stimulated the CFTR expression in thecal cell layer and granulosa cell layer of mature follicles 48 h post injection, coincident with the PMSG-induced peak in follicular estradiol. However, the expression of CFTR in the granulose lutein cell layer and thecal lutein cell layer was time-dependently reduced following hCG injection, in accordance with the gradually increased progestogen level during luteum corpus formation. Western blotting analysis demonstrated that rat ovarian tissue expressed the special CFTR band at 170 kD. It is concluded that cAMP-dependent Cl- channels are involved in regulation of follicle development and luteum formation.

引用

页码：584 / 587

页数：4

共 18 条

[1]

Hypokalemia alters sex hormone and gonadotropin levels:evidence that FSH may be required for luteinization. Tejada F,Cremades A,Aviles M et al. Endocrino Metab . 1998

[2]

Expression of cystic fibrosis transmembrane conductance regulator in human endometrium. Zheng X Y,Chen G A,Wang H Y. Human Reproduction . 2004

[3]

Ion transport by human endometrial epithelia in vitro. Matthews,CJ,Thomas,EJ,Redfern,CP,Hirst,BH. Human Reproduction . 1993

[4]

Human uterine fluid, examined in undiluted samples for osmolarity and the concentrations of inorganic ions, albumin,glucose, and urea. Casslen B,Nilsson B. American Journal of Obstetrics and Gynecology . 1984

[5]

Identifica-tion of the cystic fibrosis gene:cloning and characteriza-tion of complementary DNA. Riordan J R,Rommens J M,Kerem B S et al. Science . 1989

[6]

Cystic fibrosis. Welsh M J,Smith A E. Scientific American . 1995

[7]

Down-regulation by progesterone of CFTR expression in endometrial epithe-lial cells:a study by competitive RT-PCR. Mularoni A,Beck L,Sadir R et al. Biochemical and Biophysical Research Communications . 1995

[8]

Differential cellular expression of cystic fibrosis transmembrane regulator in human reproductive tissue. Tizzano EF,Silver MM,Chitayat D,et al. American Journal of Pathology . 1994

[9]

CFTR as a cAMP-dependent regulator of sodium channels. Stutts M J,Canessa C M,Olsen J C,et al. Science . 1995

[10]

The cystic fibrosis transmembrane conductance regulator activates aquaporin 3 in airway epithelial cells. Schreiber-R,,Nitschke-R,Greger-R ,et al. Biochemical Journal . 1999

← 1 2 →