Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic

被引：0

作者：

曹国珍 ^{[1
,2
,3
]}

张苗苗 ^{[1
,3
]}

李文建 ^{[1
,2
,3
]}

缪建顺 ^{[1
,3
]}

陆栋 ^{[1
,2
,3
]}

张文德 ^{[4
]}

机构：

[1] Institute of Modern Physics, Chinese Academy of Sciences

[2] School of Pharmacy, Lanzhou University

[3] Key Laboratory of Microbial Resources Exploitation and Application

[4] Gansu Ronghua Industry Group Co Ltd

来源：

NuclearScienceandTechniques | 2015年 / 26卷 / 06期

关键词：

X-rays; Saccharomyces cerevisiae; Growth curve; Physiological-biochemical characteristic;

D O I：

10.13538/j.1001-8042/nst.26.060302

中图分类号：

Q937 [微生物生物物理学]; O434.1 [X射线];

学科分类号：

071011 ; 070207 ; 0803 ;

摘要：

In this paper, the growth curves of yeast cells exposed to X-rays were detected, and then fitted by Gompertz equation. The yeast cells treated with 50–125 Gy showed an increased exponential growth rate, and lower total biomass at plateau. At doses ≥ 150 Gy, cells showed a decreased exponential growth rate and higher total biomass at plateau. DNA lesions were detected by comet assay. Meanwhile, intracellular accumulation of reactive oxygen species(ROS), reduction of mitochondrial membrane potential(?Ψ m) and cell membrane integrity were evaluated. We conclude that X-ray irradiation results in DNA lesions, ROS accumulation and?Ψ m decline in a dose-dependent manner, and that these changes may be one of causes of X-rays-induced apoptosis in yeast. Furthermore, yeast cell membrane integrity appeared compromised following irradiation,suggesting that membrane damage may also have a role in the biological effects of radiation.

引用

页码：48 / 56

页数：9

共 23 条

[11]

Plasma membrane signaling induced by ionizing radiation[J] . Isabelle Corre,Colin Niaudet,Francois Paris.Mutation Research-Reviews in Mutation Research . 2010 (1)

[12]

Delayed repair of radiation induced clustered DNA damage: Friend or foe?[J] . Laura J. Eccles,Peter O’Neill,Martine E. Lomax.Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis . 2010 (1)

[13]

DNA mismatch repair and the DNA damage response to ionizing radiation: Making sense of apparently conflicting data[J] . Lynn M. Martin,Brian Marples,Mary Coffey,Mark Lawler,Thomas H. Lynch,Donal Hollywood,Laure Marignol.Cancer Treatment Reviews . 2010 (7)

[14] Reactive oxygen species and yeast apoptosis [J].

Perrone, Gabriiel G. ;

Tan, Shi-Xiong ;

Dawes, Ian W. .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2008, 1783 (07) :1354-1368

[15]

Behaviour of Saccharomyces cerevisiae wine strains during adaptation to unfavourable conditions of fermentation on synthetic medium: Cell lipid composition, membrane integrity, viability and fermentative activity[J] . Ilaria Mannazzu,Daniele Angelozzi,Simona Belviso,Marilena Budroni,Giovanni Antonio Farris,Paola Goffrini,Tiziana Lodi,Mario Marzona,Laura Bardi.International Journal of Food Microbiology . 2007 (1)

[16] Hyperosmotic stress induces metacaspase- and mitochondria-dependent apoptosis in Saccharomyces cerevisiae [J].

Silva, RD ;

Sotoca, R ;

Johansson, B ;

Ludovico, P ;

Sansonetty, F ;

Silva, MT ;

Peinado, JM ;

Côrte-Real, M .

MOLECULAR MICROBIOLOGY, 2005, 58 (03) :824-834

[17] Impact of ionizing radiation on the life cycle of Saccharomyces cerevisiae Ty1 retrotransposon [J].

Sacerdot, C ;

Mercier, G ;

Todeschini, AL ;

Dutreix, M ;

Springer, M ;

Lesage, P .

YEAST, 2005, 22 (06) :441-455

[18]

Metabolic oxidation/reduction reactions and cellular responses to ionizing radiation: A unifying concept in stress response biology[J] . Douglas R. Spitz,Edouard I. Azzam,Jian Jian Li,David Gius.Cancer and Metastasis Reviews . 2004 (3)

[19]

Radiolysis of water: a look at its origin and occurrence in the nature[J] . Radiation Physics and Chemistry . 2004 (2)

[20]

Bax expression protects yeast plasma membrane against ethanol-induced permeabilization[J] . Esther Marza,Nadine Camougrand,Stéphen Manon.FEBS Letters . 2002 (1)

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