Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic

被引：0

作者：

曹国珍 ^{[1
,2
,3
]}

张苗苗 ^{[1
,3
]}

李文建 ^{[1
,2
,3
]}

缪建顺 ^{[1
,3
]}

陆栋 ^{[1
,2
,3
]}

张文德 ^{[4
]}

机构：

[1] Institute of Modern Physics, Chinese Academy of Sciences

[2] School of Pharmacy, Lanzhou University

[3] Key Laboratory of Microbial Resources Exploitation and Application

[4] Gansu Ronghua Industry Group Co Ltd

来源：

Nuclear Science and Techniques | 2015年 / 26卷 / 06期

关键词：

X-rays; Saccharomyces cerevisiae; Growth curve; Physiological-biochemical characteristic;

D O I：

10.13538/j.1001-8042/nst.26.060302

中图分类号：

Q937 [微生物生物物理学]; O434.1 [X射线];

学科分类号：

071011 ; 070207 ; 0803 ;

摘要：

In this paper, the growth curves of yeast cells exposed to X-rays were detected, and then fitted by Gompertz equation. The yeast cells treated with 50–125 Gy showed an increased exponential growth rate, and lower total biomass at plateau. At doses ≥ 150 Gy, cells showed a decreased exponential growth rate and higher total biomass at plateau. DNA lesions were detected by comet assay. Meanwhile, intracellular accumulation of reactive oxygen species(ROS), reduction of mitochondrial membrane potential(?Ψ m) and cell membrane integrity were evaluated. We conclude that X-ray irradiation results in DNA lesions, ROS accumulation and?Ψ m decline in a dose-dependent manner, and that these changes may be one of causes of X-rays-induced apoptosis in yeast. Furthermore, yeast cell membrane integrity appeared compromised following irradiation,suggesting that membrane damage may also have a role in the biological effects of radiation.

引用

页码：48 / 56

页数：9

共 23 条

[1] 分光光度法测定酿酒酵母细胞悬液浓度研究 [J].

曹国珍 ;

缪建顺 ;

张苗苗 ;

王菊芳 ;

李文建 ;

陆栋 .

中国酿造, 2014, 33 (04) :129-133

[2]

Effects of microwave irradiation dose and time on Yeast ZSM-001 growth and cell membrane permeability[J] . Shu-Wei Zeng,Qi-Lin Huang,Si-Ming Zhao.Food Control . 2014

[3] EFFECTS OF PULSED ELECTRIC FIELDS ON PHYSICOCHEMICAL PROPERTIES AND MICROBIAL INACTIVATION OF CARROT JUICE [J].

Xiang, Bob ;

Sundararajan, Srijanani ;

Solval, Kevin Mis ;

Espinoza-Rodezno, Luis ;

Aryana, Kayanush ;

Sathivel, Subramaniam .

JOURNAL OF FOOD PROCESSING AND PRESERVATION, 2014, 38 (04) :1556-1564

[4] Ionizing irradiation-induced radical stress stalls live meiotic chromosome movements by altering the actin cytoskeleton [J].

Illner, Doris ;

Scherthan, Harry .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (40) :16027-16032

[5] Oxidative Stress and Replication-Independent DNA Breakage Induced by Arsenic in Saccharomyces cerevisiae [J].

Litwin, Ireneusz ;

Bocer, Tomasz ;

Dziadkowiec, Dorota ;

Wysocki, Robert .

PLOS GENETICS, 2013, 9 (07)

[6] Effect of low-dose microwave radiation on Aspergillus parasiticus [J].

Fang, Yanpeng ;

Hu, Jian ;

Xiong, Shanbai ;

Zhao, Siming .

FOOD CONTROL, 2011, 22 (07) :1078-1084

[7]

Role of oxidative stress and DNA damage in human carcinogenesis[J] . Thomas B. Kryston,Anastassiya B. Georgiev,Polycarpos Pissis,Alexandros G. Georgakilas.Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis . 2011 (1-2)

[8]

Radiation Induced Non-targeted Response: Mechanism and Potential Clinical Implications[J] . Tom K. Hei,Hongning Zhou,Yunfei Chai,Brian Ponnaiya,Vladimir N. Ivanov.Current Molecular Pharmacology . 2011 (2)

[9]

Role of DNA Damage and Epigenetic DNA Methylation Changes in Radiation-Induced Genomic Instability and Bystander Effects in Germline In Vivo[J] . Jan Tamminga,Olga Kovalchuk.Current Molecular Pharmacology . 2011 (2)

[10]

Measuring oxidative DNA damage and DNA repair using the yeast comet assay[J] . Yeast . 2010 (1)

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