Test Method for Evaluating the Cleaning Performance of Proteases in Automatic Dishwashing Detergent

A test method was developed to better evaluate the cleaning performance of protease in Chinese automatic dishwashing detergent（ADD） and differentiate the washing performance of different ADD formulations on protein stains. Steamed egg stain, which was a Chinese-consumers-relevant soil, was used as monitor. The response curves of the stain to different amounts of protease were measured under laboratory conditions, and the stain removal ability of commercially available ADDs was tested with this method. The results showed that the soil removal rate of ADD without protease was less than 10%, and the soil removal rate increased with the increase of protease dosage in the range of 0.0%. Steamed egg stain had a good response to protease, and it could effectively distinguish the cleaning performance of commercial ADDs. The test method could be used in the development, screening and evaluation of ADD formulations.