Chemical Modification of Amino Acid Residues in Human Plasminogen

被引：0

作者：

LIANG Fang SUN Hong ZHAO Chengguang CUI Ting HONG Shuisheng CHEN Jia and LIU Lanying College of Life Science Jilin University Changchun P R China Changchun Institute of Biological Products Changchun P R China ^{[1
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机构：

来源：

Chemical Research in Chinese Universities | 2003年 / 03期

关键词：

Human plasminogen(HPg); Chemical modification; Carboxyl group; Methionine; Tryptophan;

D O I：

暂无

中图分类号：

O621 [有机化学一般性问题];

学科分类号：

070303 ; 081704 ;

摘要：

The chemical modification of human plasminogen(HPg) was studied with 1-ethyl-3-(3- dimethyl aminopropyl) carbodiimide(EDC), N -acetylimidazole(NAI), 1,2-cyclohexanedione(CHD), chloramine T(Ch-T) and N -bromosuccinimide(NBS) as modifying reagents at its carboxyl group, tyrosine, arginine, methionine and tryptophan residues, respectively. The results indicate that tyrosine and arginine residues are not essential for HPg activity, while carboxyl groups, methionine and tryptophan residues are important for the activity of HPg. The Keech and Farrant′s kinetic analysis reveals that one tryptophan residue, one methionine residue and two carboxyl groups are essential for HPg activity.

引用

页码：317 / 319

页数：3

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