Determination of IgA,IgG,IgM Class-Specific Rheumatoid Factor and Its Clinical Evaluation

被引：0

作者：

李晓军

武建国

机构：

[1] Second Military Medical University

[2] Nanjing 210002

[3] Shanghai 200433 and Department of Clinical Immunology

[4] the Department of Clinical Immunology of General Hospital of Nanjing Command of PLA Nanjing

来源：

Journal of Medical Colleges of PLA | 1989年 / 04期

关键词：

rheumatoid factor; ELISA; rheumatoid arthritis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Solid Phase enzyme-linked Immunosorbent assay(ELISA) for detectingclass-specific rheumatoid factor (RF) was established.Aggregated rabbit lgG was used ascoating antigen and the presence of RF was demonstrated by F(ab’)fragment of anti-humanIg conjugated to horseradish peroxidase(HRP).The results showed that high levels ofIgM-RF,IgG-RF and IgA-RF were found in patients with rheumatoid arthritis.A positive cor-relation existed between IgM-,IgG-,IgA-RF and disease activity.The presence of vasculitis al-so correlated positively with the levels of 3 class-specific RFs.

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页码：361 / 364

页数：4

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