miR-767-5p通过调控TBL1XR1蛋白表达抑制人卵巢癌细胞系侵袭

被引：0

作者：

冷海娜 ^{[1
]}

张艳 ^{[1
]}

王晓栋 ^{[2
]}

张学友 ^{[2
]}

李洪利 ^{[3
]}

机构：

[1] 潍坊市妇幼保健院妇科

[2] 潍坊医学院生命科学与技术学院

[3] 潍坊医学院基础医学院

来源：

基础医学与临床 | 2023年 / 43卷 / 03期

关键词：

卵巢癌; 侵袭; miR-767-5p; 转导素β1X连锁受体蛋白1(TBL1XR1);

D O I：

10.16352/j.issn.1001-6325.2023.03.408

中图分类号：

R737.31 [卵巢肿瘤];

学科分类号：

摘要：

目的探索miR-767-5p和转导素β1X连锁受体蛋白1(TBL1XR1)对卵巢癌进展的影响以及作用机制。方法数据库分析：使用GEO、miRPATH数据库和RStudio对miR-767-5p进行KEGG(京都百科全书)富集分析，并在GEO数据集低表达和高表达microRNA中各选择差异表达倍数TOP5的microRNAs,按照差异倍数由低到高排列；miRDB等数据库预测miR-767-5p可结合的靶基因并取交集，结合Open Target Platform、GEPIA等数据库筛选出目的基因；GEPIA、THPA数据库分析TBL1XR1在人体各器官表达量分析、免疫组化分析以及生存曲线分析。实验验证：双荧光素酶报告基因实验验证miR-767-5p和TBL1XR1存在结合靶点；Western blot检测卵巢癌细胞系(OC3、SKOV-3、HO-8910)和正常卵巢上皮细胞系(IOSE80)中TBL1XR1的表达；在SKOV-3细胞中转入miR-767-5p过表达质粒与TBL1XR1过表达质粒后，Western blot检测TBL1XR1表达量，Tanswell小室法检测细胞侵袭。结果数据库分析：选择低表达差异倍数最高的miR-767-5p作为目的microRNA进行后续研究；miR-767-5p参与多种癌的相关通路，且miR-767-5p、TBL1XR1与乳腺癌、卵巢癌等联系密切；TBL1XR1在卵巢癌中呈现高表达，且与患者预后不良相关。实验验证：miR-767-5p和TBL1XR1可靶向结合(P<0.05);相对于IOSE80细胞，TBL1XR1在OC3、SKOV-3、HO-8910细胞中呈现明显高表达；转入miR-767-5p过表达质粒后，TBL1XR1表达量降低，卵巢癌细胞侵袭性降低(P<0.05),而同时转入TBL1XR1过表达质粒后可在一定程度上减弱这种影响。结论 miR-767-5p通过调控TBL1XR1的表达抑制人卵巢癌细胞系的侵袭。

引用

页码：408 / 414

页数：7

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