A selective coumarin-based “turn-on” fluorescent sensor for the detection of cysteine and its applications for bioimaging

被引：0

作者：

Yan Yang ^{[1
]}

Huan Wang ^{[2
]}

YuLin Wei ^{[3
]}

Jie Zhou ^{[1
]}

JunFeng Zhang ^{[2
]}

Ying Zhou ^{[1
]}

机构：

[1] College of Chemical Science and Technology, Yunnan University

[2] College of Chemistry and Chemical Engineering, Yunnan Normal University

[3] Department of Safety Evaluation, Technology Center of China Tobacco Yunnan Industrial Co, Ltd

来源：

ChineseChemicalLetters | 2017年 / 28卷 / 10期

关键词：

Fluorescence; Sensor; Cysteine; Coumarin; In vivo imaging;

D O I：

暂无

中图分类号：

O657.3 [光化学分析法（光谱分析法）]; TP212 [发送器（变换器）、传感器];

学科分类号：

070302 ; 081704 ; 080202 ;

摘要：

A coumarin-based compound（1） was designed and synthesized as a new turn-on fluorescent probe for the detection of cysteine. The probe exhibited higher selectivity towards the target molecule over other thiol and amino acids at pH 7.2 in aqueous media CH3CN-HEPES（0.02 mol/L, pH 7.2, 1:9, v/v）. The reaction mechanism is attributed to the cysteine-induced SNAr substitution-rearrangement reaction. Remarkable enhancement of up to 20-fold in fluorescence intensity was achieved in the detection of cysteine. When applied for the fluorescence imaging of cysteine, the compound 1 emitted a green fluorescence in Hi5 cell cytoplasm. The in vivo imaging of Caenorhabditis elegans had further confirmed the cysteine detection by compound 1.

引用

页码：2023 / 2026

页数：4