血管新生对局灶性脑缺血再灌注后脑室下区神经干细胞增殖的影响

被引：2

作者：

李志宁 ^{[1
]}

韩卫 ^{[2
]}

荣良群 ^{[1
]}

龚爱平 ^{[1
]}

吕尤 ^{[1
]}

单君君 ^{[1
]}

魏秀娥 ^{[1
]}

机构：

[1] 徐州医科大学第二附属医院神经内科

[2] 徐州医科大学第二附属医院检验科

来源：

中国组织工程研究 | 2017年 / 21卷 / 29期

关键词：

神经干细胞; 脑缺血; 血管内皮生长因子类; 细胞增殖; 组织工程; 干细胞; 线栓法; 局灶性脑缺血; 脑室下区; 血管新生;

D O I：

暂无

中图分类号：

R743 [脑血管疾病];

学科分类号：

1002 ;

摘要：

背景:血管新生可能与神经干细胞的增殖有相关性,但仍无统一观点。目的:观察血管新生对局灶性缺血再灌注后大鼠脑室下区神经干细胞增殖的影响。方法:成年雄性SD大鼠,随机分为正常组、假手术组、手术组、侧脑室注射血管内皮生长因子+手术组、侧脑室注射生理盐水+手术组,各组再分为缺血再灌注1,3,7,14 d组,每组12只。线栓法制作局部脑缺血再灌注模型,给予相应干预措施,利用免疫组化法检测各组大鼠脑室下区Nestin的表达,Real time-PCR法检测各组大鼠脑室下区vWF mRNA的表达。结果与结论:①缺血再灌注后脑室下区vWF mRNA、Nestin阳性表达增加;在缺血后7 d,vWF mRNA、Nestin阳性表达均达高峰,提示局灶性缺血再灌注后大鼠脑室下区神经干细胞的增殖与血管新生存在时间相关性;②侧脑室注射血管内皮生长因子+手术组vWF mRNA、Nestin阳性表达较手术组及侧脑室注射生理盐水+手术组明显增高,提示缺血再灌注后血管新生可促进大鼠脑室下区神经干细胞的增殖。

引用

页码：4697 / 4702

页数：6

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