Prediction of promiscuous T-cell epitopes in the Zika virus polyprotein:An in silico approach

被引：0

作者：

Hamza Dar ^{[1
]}

Tahreem Zaheer ^{[1
]}

Muhammad Talha Rehman ^{[1
]}

Amjad Ali ^{[1
]}

Aneela Javed ^{[1
]}

Gohar Ayub Khan ^{[1
]}

Mustafeez Mujtaba Babar ^{[2
]}

Yasir Waheed ^{[3
]}

机构：

[1] Atta-ur-Rahman School of Applied Biosciences(ASAB),National University of Sciences and Technology

[2] Shifa College of Pharmaceutical Sciences,Shifa Tameer-e-Millat University 3. Foundation University Medical College,Foundation University Islamabad

来源：

Asian Pacific Journal of Tropical Medicine | 2016年 / 09期

关键词：

Zika Virus; B-Cell Epitopes; T-Cell Epitopes; Vaccine; Antigenicity;

D O I：

暂无

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Objective:To predict immunogenic promiscuous T-cell epitopes from the polyprotein of the Zika virus using a range of bioinformatics tools.To date,no epitope data are available for the Zika virus in the IEDB database.Methods:We retrieved nearly 54 full length polyprotein sequences of the Zika virus from the NCBI database belonging to different outbreaks.A consensus sequence was then used to predict the promiscuous T cell epitopes that bind MHC 1 and MHC II alleles using Propred1 and Propred immunoinformatic algorithms respectively.The antigencity predicted score was also calculated for each predicted epitope using the Vaxi Jen 2.0 tool.Results:By using Pro Pred1,23 antigenic epitopes for HLA class I and 48 antigenic epitopes for HLA class II were predicted from the consensus polyprotein sequence of Zika virus.The greatest number of MHC class I binding epitopes were projected within the NS5(21%),followed by Envelope(17%).For MHC class II,greatest number of predicted epitopes were in NS5(19%) followed by the Envelope,NS1 and NS2(17% each).A variety of epitopes with good binding affinity,promiscuity and antigenicity were predicted for both the HLA classes.Conclusion:The predicted conserved promiscuous T-cell epitopes examined in this study were reported for the first time and will contribute to the imminent design of Zika virus vaccine candidates,which will be able to induce a broad range of immune responses in a heterogeneous HLA population.However,our results can be verified and employed in future efficacious vaccine formulations only after successful experimental studies.

引用

页码：822 / 828

页数：7