Rapid Internal Control Reference Recombinase-Aided Amplification Assays for EBV and CMV Detection

被引:0
|
作者
GAO Yuan [1 ,2 ,3 ]
TIE Yan Qing [3 ]
ZHAO Lin Qing [4 ]
TAN He [1 ,3 ]
DING Nan [3 ]
DING Ya Xin [4 ]
GUO Qi [4 ]
ZHANG Rui Qing [2 ]
WANG Jin Rong [1 ,2 ]
CHEN Zi Wei [2 ,5 ]
FAN Guo Hao [2 ]
SHEN Xin Xin [2 ]
FENG Zhi Shan [3 ]
MA Xue Jun [2 ,6 ]
机构
[1] Hebei Medical University
[2] NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention
[3] Hebei General Hospital
[4] Laboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics
[5] The Third Xiangya Hospital of Central South University
[6] Center for Biosafety Mega-Science,Chinese Academy of Sciences
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中图分类号
R440 [];
学科分类号
100208 ;
摘要
Epstein-Barr virus (EBV) and cytomegalovirus(CMV), two of the most prevalent human herpesviruses, cause a wide spectrum of diseases and symptoms and are associated with serious health problem. In this study, we developed an internal control reference recombinase-aided amplification (ICR-RAA) assay for the rapid detection of EBV and CMV within 30 min. The assay had a sensitivity of 5 and 1 copies/test for EBV and CMV,
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页码:650 / 655
页数:6
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