Troglitazone induced apoptosis of human pterygium fibroblasts through a mitochondrial-dependent pathway

被引：0
|
作者：
Xiao-Xi Yang 1
机构：
来源：
International Journal of Ophthalmology | 2011年 / 02期
基金：
中国国家自然科学基金;
关键词：
pterygium; peroxisome proliferator-activated receptor γ; troglitazone; apoptosis;
D O I：
暂无
中图分类号：
R965 [实验药理学];
学科分类号：
100602 ; 100706 ;
摘要：
·AIM:To study the effect of troglitazone on primary culture human pterygium fibroblasts (HPF).·METHODS:Cell viability loss and apoptosis were quantified by cell counting kit-8,AnnexinV-FITC/PI double staining,caspases activity test and western blotting.Flow cytometry was used to detect mitochondrial membrane potential.·RESULTS:Peroxisome proliferator-activated receptor γ (PPAR-γ) was positively expressed in pterygium specimens (n=5).Troglitazone showed dose-dependent inhibition of cell survival,induced phospholipids redistribution,activated caspase-3,-9,and altered mitochondrial potential.Western blot assay demonstrated the increase of Bax/Bcl-2 protein ratio.·CONCLUSION:Troglitazone induced apoptosis of HPF through a mitochondrial-dependent pathway.·
引用
收藏
页码：143 / 146
页数：4
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