miR-590-5p通过靶向抑制YAP1的表达抑制A375恶性黑素瘤细胞侵袭和迁移

被引：4
|
作者：
王丽娟 [1 ]
史圣甲 [2 ]
周艳 [1 ]
穆欣 [1 ]
韩丹 [1 ]
葛睿 [1 ]
牟宽厚 [1 ]
机构：
[1] 西安交通大学第一附属医院皮肤科
[2] 第四军医大学西京医院泌尿外科
来源：
细胞与分子免疫学杂志 | 2017年 / 03期
关键词：
miR-590-5p; YAP1; 侵袭; 迁移; 恶性黑素瘤;
D O I：
10.13423/j.cnki.cjcmi.008072
中图分类号：
R739.5 [皮肤肿瘤];
学科分类号：
摘要：
目的探索miR-590-5p在A375恶性黑素瘤细胞中的表达及其对A375细胞侵袭和迁移能力的影响和机制。方法应用实时定量PCR技术检测A375细胞与人正常黑素细胞中miR-590-5p的表达水平;应用TranswellTM实验和划痕实验检测miR-590-5p对A375细胞侵袭和迁移的影响;应用荧光素酶报告基因实验明确yes相关蛋白(YAP1)是否为miR-590-5p的直接靶分子;应用实时定量PCR及Western blot法检测miR-590-5p对YAP1表达水平的影响。结果 miR-590-5p在A375细胞中的表达水平显著低于正常黑素细胞;与对照(miR-590-5p-NC)组相比,miR-590-5p mimcs组细胞侵袭和迁移能力均受到显著抑制;YAP1为miR-590-5p的直接靶分子;与miR-590-5p-NC组相比,miR-590-5p mimcs组细胞中YAP1 mRNA及蛋白的表达均受到抑制。结论 miR-590-5p在A375细胞中低表达,并通过靶向抑制YAP1的表达调控A375细胞的侵袭和迁移能力。
引用
收藏
页码：326 / 330+336 +336
页数：6
相关论文
共 17 条
  • [1] miR-590-5p和miR-590-3p参与肝细胞肝癌发展的机制[J]. 杨红飞,郑文宏,赵文淘,关春燕,安靓.南方医科大学学报. 2013(06)
  • [2] MicroRNAs role as potential biomarkers and key regulators in melanoma
    Varamo, Chiara
    Occelli, Marcella
    Vivenza, Daniela
    Merlano, Marco
    Lo Nigro, Cristiana
    [J]. GENES CHROMOSOMES & CANCER, 2017, 56 (01): : 3 - 10
  • [3] miRNA expression profile of vulvar squamous cell carcinoma and identification of the oncogenic role of miR-590-5p
    Yang, Xiuhua
    Wu, Xin
    [J]. ONCOLOGY REPORTS, 2016, 35 (01) : 398 - 408
  • [4] miRNAs in Melanoma: Tumor Suppressors and Oncogenes with Prognostic Potential[J] . Michal Wozniak,Aleksandra Mielczarek,Malgorzata Czyz.Current Medicinal Chemistry . 2016 (28)
  • [5] Cancer statistics in China, 2015[J] . Wanqing Chen,Rongshou Zheng,Peter D. Baade,Siwei Zhang,Hongmei Zeng,Freddie Bray,Ahmedin Jemal,Xue Qin Yu,Jie He.CA: A Cancer Journal for Clinicians . 2016 (2)
  • [6] Cancer statistics, 2016[J] . Rebecca L. Siegel,Kimberly D. Miller,Ahmedin Jemal.CA: A Cancer Journal for Clinicians . 2016 (1)
  • [7] The Hippo effector YAP regulates the response of cancer cells to MAPK pathway inhibitors
    Lin, Luping
    Bivona, Trever G.
    [J]. MOLECULAR & CELLULAR ONCOLOGY, 2016, 3 (01):
  • [8] YAP and TAZ: a nexus for Hippo signaling and beyond[J] . Carsten Gram Hansen,Toshiro Moroishi,Kun-Liang Guan.Trends in Cell Biology . 2015 (9)
  • [9] Genetic variants in Hippo pathway genes YAP 1, TEAD 1 and TEAD 4 are associated with melanoma‐specific survival[J] . Hua Yuan,Hongliang Liu,Zhensheng Liu,Dakai Zhu,Christopher I. Amos,Shenying Fang,Jeffrey E. Lee,Qingyi Wei.Int. J. Cancer . 2015 (3)
  • [10] MicroRNAs as Potential Biomarkers in Cancer: Opportunities and Challenges[J] . Huiyin Lan,Haiqi Lu,Xian Wang,Hongchuan Jin,Francesco Nicassio.BioMed Research International . 2015
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