Study on Antibacterial Activities of Crude Extracts from the Whole Herb of Orostachys cartilaginous A. Bor in Vitro

[Objective] To study antibacterial activities of crude extracts from the whole herb of Orostachys cartilaginous A.Bor in vitro. [Method] liquid-liquid extraction technique was applied to isolate active ingredients from 0.75 acetone (volume fraction) extracts for preparing chloroform, ethyl acetate, normal butanol and water phases. The antibacterial activities of extraction phases against 4 tested strains in vitro were investigated by combining filter paper method with double dilution method. [Result] Extraction phases from 0.75 acetone extracts had antibacterial activities against Staphyloccocus aureus Rosenbach, Bacillus subtilis (Ehrenberg) Cohn, Escherichia coli and Proteus vulgaris in varying degrees. Chloroform and ethyl acetate extraction phases were provided with strong antibacterial activities against Staphylococcus aureus with minimal inhibitory concentration of 1.25 mg/ml. [Conclusion] Active ingredients of Orostachys cartilaginous A.Bor plant were mainly existed in chloroform and ethyl acetate phases, which could provide a scientific basis for developing antibacterial agents from Orostachys cartilaginous A.Bor.