MicroRNA-34a通过靶基因c-Met抑制施万细胞增殖

被引:0
|
作者
李石营
陈褚
刘倩倩
王星辉
冯倩
丁斐
顾晓松
机构
[1] 南通大学江苏省神经再生重点实验室
关键词
microRNA-34a; c-Met; 施万细胞; 增殖;
D O I
暂无
中图分类号
R329.28 [];
学科分类号
摘要
目的:探讨坐骨神经横断后,microRNA-34a(miR-34a)对c-Met(肝细胞生长因子受体)的调控作用及对施万细胞增殖的影响。方法:采用Real-time PCR检测miR-34a和c-Met在坐骨神经横断后近侧端的表达变化;Edu染色法检测miR-34a对原代培养的施万细胞增殖能力的影响;双荧光素酶报告基因系统检测miR-34a对c-Met 3’UTR的直接靶向作用。结果:miR-34a与c-Met在坐骨神经横断后近侧端的表达呈负相关,能够抑制原代培养的施万细胞的增殖,并可直接作用于c-Met基因的3’UTR。结论:miR-34a可以直接靶向c-Met的3’UTR,从而通过下调靶基因c-Met的表达抑制施万细胞的增殖。
引用
收藏
页码:443 / 446
页数:4
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共 13 条
  • [1] Identification and functional annotation of novel microRNAs in the proximal sciatic nerve after sciatic nerve transection[J]. LI ShiYing , YU Bin , WANG YongJun, YAO DengBing, ZHANG ZhanHu & GU XiaoSong * Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 210063, China.  Science China(Life Sciences). 2011(09)
  • [2] Identification and functional analysis of novel micro-RNAs inrat dorsal root ganglia after sciatic nerve resection. Li S,Yu B,Wang S. Journal of Neuroscience Research . 2012
  • [3] Profile of microRNAs following rat sciatic nerve injury by deep sequencing: implication for mechanisms of nerve regeneration. Yu B,Zhou S,Wang Y,et al. PLoS One . 2011
  • [4] miR-221/222 promoteSchwann cell proliferation and migration by targeting LASS2following sciatic nerve injury. Yu B,Zhou S,Wang Y,et al. Journal of Cell Science . 2012
  • [5] Real-time quantification of microRNAs by stem-loop RT-PCR. Chen C,Ridzon D A,Broomer A J, et al. Nucleic Acids Research . 2005
  • [6] Identification of many microRNAs that copurify with polyribosomes in mammalian neurons. Kim J,Krichevsky A,Grad Y, et al. Proceedings of the National Academy of Sciences of the United States of America . 2004
  • [7] Identification and quantitative Analyses of microRNAs located in the distal axons of sympathetic neurons. Natera M0,Aschrafi A,Gioio AE, et al. Rádio Nacional de Angola . 2010
  • [8] MicroRNAs in vertebrate synapse development. Fiore R,Schratt G. Sci World J . 2007
  • [9] microRNAs at the synapse. Schratt G. Nature Reviews Neuroscience . 2009
  • [10] Characteriza-tion of human transforming genes from chemically trans-formed,teratocarcinoma,and Pancreatic carcinoma cell lines. Cooper CS,Blair DG,Oskarsson MK,et al. Cancer Research . 1984