Isolation, Identification and Pathogenicity Evaluation of Pathogens of Soybean Bacterial Diseases

被引：0

作者：

Zhang Hui

Yu Renjing

Zou Jianan

Xin Dawei

Hu Zhenbang

Zhang Yong

Liu Chunyan

Chen Qingshan

机构：

[1] College of Agriculture,Northeast Agricultural University

[2] Keshan Branch of Heilongjiang Academy of Agricultural Sciences

[3] Land Reclamation Research and Breeding Center of Heilongjiang

来源：

Plant Diseases and Pests | 2016年 / Z1期

基金：

中国国家自然科学基金;

关键词：

Soybean; Bacterial pathogen; Isolation; Identification; Pathogenicity;

D O I：

10.19579/j.cnki.plant-d.p.2016.z1.003

中图分类号：

S435.651 [大豆病虫害];

学科分类号：

090401 ; 090402 ;

摘要：

In order to research soybean bacterial diseases in Heilongjiang Province,a bacterial strain was isolated from diseased leaves of Suinong 14 collected from Harbin Yanjiagang farm. The pathogen had been carried out antibiotic resistance identification,molecular identification,re-inoculation identification and pathogenicity identification respectively. By Gram staining,pathogen inoculation test,16 S r DNA molecular identification,the pathogen was determined as Pseudomonas syringae and named Psgneau 2. The isolated strain presented resistance to 25,50 and 100 μg/m L ampicillin and carbenicillin,but susceptible to spectinomycin,rifampicin,kanamycin,gentamicin,tetracycline and chloramphenicol. Ten soybean varieties were inoculated with Psgneau 2,in which Suinong 14 was more susceptible than other varieties and Hefeng 35 presented higher resistance to Psgneau 2.

引用

页码：8 / 12

页数：5

共 16 条

[1]

Effects of tillage,cultivar,and planting date on percentage of soybean leaves with symptoms of sudden death syndrome. Wrather J A,Kendig S R,Anand S C,Niblack T L,Smith G S. Plant Disease . 1995

[2]

Inheritance of resistance to bacterial blight (Pseudomonas glycinea Coerper) in soybeans (Glycine max L.). Mukherjee,D,Lambert,JW,Cooper,RL,Kennedy,BW. Crop Science . 1966

[3]

Races of Pseudomonas syringae pv.glycinea on commercial soybean in eastern north Dakota. Prom L K,Venette J R. Plant Disease . 1997

[4]

Pathogenic races of the bacterial blight pathogen of soybeans, Pseudomonas glycinea. Cross JE,Kennedy BW,Lambert JW, et al. Plant Disease Reporter . 1966

[5]

Effects of bacterial blight on soybean yield. Park,E. W. and Lim,S. M. Plant Disease . 1986

[6]

Genomic analysis of the kiwifruit pathogen Pseudomonas syringae pv.actinidiae provides insight into the origins of an emergent plant disease. McC ann H C,Rikkerink E H A,Bertels F,et al. PLoS Pathogens . 2013

[7]

Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea. Staskawicz B,Dahlbeck D,Keen N,et al. Journal of Bacteriology . 1987

[8]

The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco. Gupta Kapuganti J,Brotman Yariv,Segu Shruthi,Zeier Tatiana,Zeier Jürgen,Persijn Stefan T,Cristescu Simona M,Harren Frans J M,Bauwe Hermann,Fernie Alisdair R,Kaiser Werner M,Mur Luis A J. Journal of Experimental Botany . 2012

[9]

Defects in D-Rhamnosyl Residue Biosynthetic Genes Affect Lipopolysaccharide Structure, Motility, and Cell-Surface Hydrophobicity in Pseudomonas syringae Pathovar glycinea Race 4[J] . Kazuhiro CHIKU,Kazuhiko TSUNEMI,Masanobu YAMAMOTO,Mayumi OHNISHI-KAMEYAMA,Mitsuru YOSHIDA,Tadashi ISHII,Fumiko TAGUCHI,Masako IWAKI,Yuki ICHINOSE,Hiroshi ONO. &nbspBioscience, Biotechnology, and Biochemistry . 2013 (3)

[10] 大豆品种资源抗细菌斑点病鉴定与评价 [J].

孙永吉 ;

刘宗麟 ;

刘玉芝 ;

胡吉成 .

大豆科学, 1989, (02) :185-189

← 1 2 →