梨抗黑星病AFLP标记筛选

被引:2
作者
董星光
方成泉
王斐
王志刚
樊丽
林盛华
机构
[1] 中国农业科学院果树研究所农业部果树种质资源利用重点开放实验室
关键词
pear scab; BSA; fluorescent AFLP; selective genotype analysis;
D O I
10.13926/j.cnki.apps.2010.05.017
中图分类号
S661.2 [梨];
学科分类号
090201 ;
摘要
Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population derived from the cross of 'Huangguan' and 'Yali' was analyzed genetically.The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner.Bulked segregant analysis(BSA) was conducted to screen 64 fluorescent AFLP primer pairs.A marker designated as D3-365 was found to be linked to the resistant locus.Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.
引用
收藏
页码:552 / 555
页数:4
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