Influence of low dietary histamine on the seizure development of chemical kindling induced by pentylenetetrazol in rats

<正> Aim:To determine the role of dietary low histamine on the seizure development ofpentylenetetrazol (PTZ)-induced kindling in rats.Methods:After 14 d of feedingon a low histamine diet (LH,containing 0.145 μmol/g of histamine),the rats werechemically kindled by repeated intraperitoneal injection of a subconvulsant doseof PTZ (35 mg/kg) once every 48 h,and seizure activity of kindling was recordedfor 30 min.Histamine in brain samples was analyzed using a high performanceliquid chromatography system with a fluorescence spectrofluorometer.Results:The LH diet induced an increase in seizure response (seizure susceptibility) to thefirst trial of PTZ,and resulted in facilitation of subsequent PTZ kindling process(seizure development).The histamine levels in the cortex,hippocampus,andhypothalamus of LH-treated rats decreased significantly and these changes cor-related well with seizure behavior (r=0.875,0.651,and 0.796,respectively).In addition,chronic kindled seizures resulted in a significant increase of the histamine contentin the cortex and hypothalamus in the LH-fed groups.Conclusion:These find-ings indicate that the histamine in daily food could influence the brain histaminer-gic function,and play an important role in regulating seizure susceptibility.