Study on Antioxidant Activity of Different Extracts of Gynura segetum in vitro

[Objectives] To study the antioxidant activity of different solvent extracts of Gynura segetum in vitro, so as to provide experimental basis for further study and utilization of medicinal resources of G. segetum. [Methods] The antioxidant activity of different solvent extracts of G. segetum was determined by UV-vis spectrophotometer using DPPH free radical scavenging method and ABTS;free radical scavenging method. [Results] The scavenging rates of DPPH and ABTS;by acetone extract were high, reaching 98.9% and 98.3%, respectively; the scavenging rate of DPPH and ABTS;by water extract was the lowest, and the scavenging rates were 76.7% and 77.5%, respectively. The order of ABTS;radical scavenging rate of samples extracted with different extractants was as follows: S;（acetone extract）>S;（ethyl acetate extract）>S;（ethanol extract）>S;（n-butanol extract）>S;（methanol extract）>S;（distilled water extract）; the order of DPPH radical scavenging rate of samples extracted with different extractants was as follows: S;（acetone extract）>S;（ethyl acetate extract）>S;（n-butanol extract） >S;（ethanol extract）>S;（methanol extract）>S;（distilled water extract）. [Conclusions] The extracts of acetone, ethyl acetate, n-butanol, anhydrous ethanol, methanol and distilled water from the roots of G. segetum had obvious scavenging effects on DPPH and ABTS;, all had antioxidant components, and acetone had the highest antioxidant effect as an extractant.