NKG2D在口腔鳞癌患者CD3(+)CD56(+)NKT细胞中的作用

被引:0
作者
孟玉生 [1 ,2 ]
杨宏宇 [1 ]
余术宜 [1 ]
杨辉俊 [1 ]
沈时岳 [1 ]
王锋 [1 ,2 ]
马平平 [1 ,2 ]
机构
[1] 北京大学深圳医院口腔颌面外科
[2] 汕头大学医学院
基金
广东省自然科学基金;
关键词
CD3(+)CD56(+)NKT细胞; NKG2D; 口腔鳞状细胞癌;
D O I
暂无
中图分类号
R739.8 [口腔、颌面部肿瘤];
学科分类号
100214 ;
摘要
目的:探讨口腔鳞癌患者CD3(+)CD56(+)NKT细胞NKG2D受体抗肿瘤免疫的调节作用。方法:分别从30例口腔鳞癌患者和30例正常人外周血中提取CD3(+)CD56(+)NKT细胞,实时定量PCR和Western印迹分析NKG2D在分子水平和蛋白水平的表达差异。通过siRNA-NKG2D转染方法,分析NKG2D受体对CD3(+)CD56(+)NKT细胞的细胞毒性,分泌IL-2和IFN-μ的影响。采用SPSS13.0软件包对数据进行t检验和方差分析。结果:口腔鳞癌患者CD3(+)CD56(+)NKT细胞NKG2D的表达显著低于正常人(P<0.05);转染siRNA-NKG2D的CD3(+)CD56(+)NKT细胞对肿瘤细胞的杀伤作用显著降低。结论:与正常人相比,口腔鳞癌患者中CD3(+)CD56(+)NKT细胞因NKG2D表达下降,其肿瘤免疫调控作用受到影响。这些发现为NKG2D应用于口腔鳞癌的免疫治疗奠定了基础。
引用
收藏
页码:275 / 280
页数:6
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