Gene Expression Profiling on Acute Rejected Transplant Kidneys with Microarray

To investigate the gene expression profiles in acute allograft rejection of renal trans-plantation,and identify the markers for the early diagnosis of acute rejection,heterotopic kidney transplantation was performed by using F344 or Lewis donors and Lewis recipients.No immunosup-pressant was used.Renal grafts were harvested on days 3,7,and 14.A commercial microarray was used to measure gene expression levels in day-7 grafts.The expression levels of 48 genes were up-regulated in the allograft in comparison with the isograft control,and interferon-γ-induced GTPase gene was most significantly up-regulated in allografts.It is concluded that a variety of pathways are involved in organ transplant rejection which is dynamic and non-balanced.IFN-inducible genes,such as IGTP,may play an important role in the rejection.A lot of important factors involved in acute re-jection are unnecessary but sufficient conditions for the rejection.We are led to conclude that it is virtually impossible to make an early diagnosis based on a single gene marker,but it could be achieved on the basis of a set of markers.