Ultrasonic Extraction Spectrophotometry Determination of Flavonoids from the Climbing Fern

This paper discusses the basic principles and characteristics of the effect of ultrasonic extraction of chemical substances from samples. With the design of orthogonal experiments, using ethanol as extractant, changing the concentration of solvent (ethanol), the time of ultrasonic assisted extraction, temperature, the extraction times and so on, we discussed the factors influencing the extraction weight and found a best condition: The ethanol concentration is 60%, the temperature is 25 ℃, the ultrasonic time is 10 min, the extraction time is 3 times. Compared with the conventional water extraction and the France extraction, the results are satisfied with the flavonoids extraction content reached 4.12%. By comparing this method with the other two, it improves the speed without heat, saves the labor, materials energy and time, so it is proved to be the efficient for extraction.