A Newly Developed Cell-free System of Peanut Germ Lysate for Protein Synthesis

被引：0
作者：
彭朝晖
杨根远
机构：
[1] Department of Biochemistry The First Medical College of PLA
[2] Guangzhou
来源：
JournalofMedicalCollegesofPLA | 1986年 / 02期
关键词：
cell-free lysate of peanut germ; protein synthesis; rat; liver; RNAs;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A peanut germ cell-free system(PS30)used for protein synthesisin vitro has been newly developed.The PS30 has a maximum ultravioletabsorbance at λ 258nm.The rates of endogenous protein synthesis of PS30 and wheat germ cell-free system(WS30)used as an experimental con-trol were 5.9±0.5 and 7.1±0.8 folds,respectively.The translationalactivity of rat liver RNAs in PS30 or WS30 was almost identical.
引用
收藏
页码：185 / 188
页数：4
相关论文
共 50 条
  • [31] Synthesis of biologically active Shiga toxins in cell-free systems
    Ramm, Franziska
    Kaser, Danny
    Koenig, Irina
    Fellendorf, Juliane
    Wenzel, Dana
    Zemella, Anne
    Papatheodorou, Panagiotis
    Barth, Holger
    Schmidt, Herbert
    [J]. SCIENTIFIC REPORTS, 2024, 14 (01)
  • [32] CTELS: A Cell-Free System for the Analysis of Translation Termination Rate
    Lashkevich, Kseniya A.
    Shlyk, Valeriya I.
    Kushchenko, Artem S.
    Gladyshev, Vadim N.
    Alkalaeva, Elena Z.
    Dmitriev, Sergey E.
    [J]. BIOMOLECULES, 2020, 10 (06) : 1 - 19
  • [33] Cell-free Protein Synthesis in a Microchamber Revealed the Presence of an Optimum Compartment Volume for High-order Reactions
    Okano, Taiji
    Matsuura, Tomoaki
    Suzuki, Hiroaki
    Yomo, Tetsuya
    [J]. ACS SYNTHETIC BIOLOGY, 2014, 3 (06): : 347 - 352
  • [34] Hollow fiber reactor for continuous flow cell-free protein production
    Yamamoto, YI
    Nagahori, H
    Yao, SL
    Zhang, ST
    Suzuki, E
    [J]. JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1996, 29 (06) : 1047 - 1050
  • [35] An integrated cell-free metabolic platform for protein production and synthetic biology
    Jewett, Michael C.
    Calhoun, Kara A.
    Voloshin, Alexei
    Wuu, Jessica J.
    Swartz, James R.
    [J]. MOLECULAR SYSTEMS BIOLOGY, 2008, 4 (1)
  • [36] Experiment and mathematical modeling of gene expression dynamics in a cell-free system
    Stoegbauer, Tobias
    Windhager, Lukas
    Zimmer, Ralf
    Raedler, Joachim O.
    [J]. INTEGRATIVE BIOLOGY, 2012, 4 (05) : 494 - 501
  • [37] Internal initiation of polyuridylic acid translation in bacterial cell-free system
    Sogorin, E. A.
    Agalarov, S. Ch.
    Spirin, A. S.
    [J]. BIOCHEMISTRY-MOSCOW, 2013, 78 (12) : 1354 - 1357
  • [38] Exploration of the Tolerance Ability of a Cell-Free Biosynthesis System to Toxic Substances
    Zhang, Peng
    Wang, Junqian
    Ding, Xuanwei
    Lin, Jun
    Jiang, Hao
    Zhou, Hongjun
    Lu, Yuan
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2019, 189 (04) : 1096 - 1107
  • [39] Cell-Free Protein Synthesis in Bifunctional Hyaluronan Microgels: A Strategy for In Situ Immobilization and Purification of His-Tagged Proteins
    Heida, Thomas
    Koehler, Tony
    Kaufmann, Anika
    Maennel, Max J.
    Thiele, Julian
    [J]. CHEMSYSTEMSCHEM, 2020, 2 (03)
  • [40] Effects of hydrostatic pressure on the activity of rat ribosome and cell-free translation system
    Lu, B
    Li, Q
    Liu, WY
    Ruan, KC
    [J]. BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, 1997, 43 (03): : 499 - 506
12345