A Newly Developed Cell-free System of Peanut Germ Lysate for Protein Synthesis

被引:0
作者
彭朝晖
杨根远
机构
[1] Department of Biochemistry The First Medical College of PLA
[2] Guangzhou
[3] Department of Biochemistry
[4] The First Medical College of PLA
关键词
cell-free lysate of peanut germ; protein synthesis; rat; liver; RNAs;
D O I
暂无
中图分类号
学科分类号
摘要
<正> A peanut germ cell-free system(PS30)used for protein synthesisin vitro has been newly developed.The PS30 has a maximum ultravioletabsorbance at λ 258nm.The rates of endogenous protein synthesis of PS30 and wheat germ cell-free system(WS30)used as an experimental con-trol were 5.9±0.5 and 7.1±0.8 folds,respectively.The translationalactivity of rat liver RNAs in PS30 or WS30 was almost identical.
引用
收藏
页码:185 / 188
页数:4
相关论文
共 50 条
[21]   The viral protein 3CD induces an equilibrium between the viral protein and RNA synthesis in a cell-free system for poliovirus replication [J].
Y. Verlinden ;
A. Cuconati ;
E. Wimmer ;
B. Rombaut .
Archives of Virology, 2002, 147 :731-744
[22]   Expression of Functional Candida antarctica Lipase B in a Cell-Free Protein Synthesis System Derived from Escherichia coli [J].
Park, Chang-Gil ;
Kim, Tae-Wan ;
Oh, In-Seok ;
Song, Jae Kwang ;
Kim, Dong-Myung .
BIOTECHNOLOGY PROGRESS, 2009, 25 (02) :589-593
[23]   Influence of cryoprotective agents on protein biosynthesis in Krebs-2 ascites carcinoma and wheat germ cell-free systems [J].
Gulevskyy, Oleksandr K. .
CRYOBIOLOGY, 2020, 96 :55-60
[24]   A Simplified and Robust Protocol for Immunoglobulin Expression in Escherichia coli Cell-Free Protein Synthesis Systems [J].
Cai, Qi ;
Hanson, Jeffrey A. ;
Steiner, Alexander R. ;
Cuong Tran ;
Masikat, Mary Rose ;
Chen, Rishard ;
Zawada, James F. ;
Sato, Aaron K. ;
Hallam, Trevor J. ;
Yin, Gang .
BIOTECHNOLOGY PROGRESS, 2015, 31 (03) :823-831
[25]   INVITRO TRANSLATION IN A HAMSTER BRAIN CELL-FREE SYSTEM [J].
THERIAUL, GR ;
GAUTHIER, D .
JOURNAL OF NEUROSCIENCE METHODS, 1991, 37 (03) :191-198
[26]   A highly efficient human cell-free translation system [J].
Aleksashin, Nikolay A. ;
Chang, Stacey Tsai-Lan ;
Cate, Jamie H. D. .
RNA, 2023, 29 (12) :1960-1972
[27]   Cell-free synthesis system-assisted pathway bottleneck diagnosis and engineering in Bacillus subtilis [J].
Tian, Rongzhen ;
Wang, Minghu ;
Shi, Jintian ;
Qin, Xiaolong ;
Guo, Haoyu ;
Jia, Xuanjie ;
Li, Jianghua ;
Liu, Long ;
Du, Guocheng ;
Chen, Jian ;
Liu, Yanfeng .
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, 2020, 5 (03) :131-136
[28]   Evidence That Phosphorylation of the α-Subunit of eIF2 Does Not Essentially Inhibit mRNA Translation in Wheat Germ Cell-Free System [J].
Zhigailov, Andrey, V ;
Alexandrova, Alena M. ;
Nizkorodova, Anna S. ;
Stanbekova, Gulshan E. ;
Kryldakov, Ruslan, V ;
Karpova, Oxana, V ;
Polimbetova, Nailya S. ;
Halford, Nigel G. ;
Iskakov, Bulat K. .
FRONTIERS IN PLANT SCIENCE, 2020, 11
[29]   INCORPORATION OF [C-14] LEUCINE INTO HUMAN SKELETAL-MUSCLE PROTEIN IN A CELL-FREE SYSTEM AS A MEASURE OF PROTEIN-SYNTHESIS - INFLUENCE OF STRESS HORMONES [J].
WERNERMAN, J ;
VONDERDECKEN, A ;
HAMMARKVIST, F ;
BOTTA, D ;
VINNARS, E .
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 1990, 9 (01) :1-9
[30]   The growing impact of lyophilized cell-free protein expression systems [J].
Hunt, J. Porter ;
Yang, Seung Ook ;
Wilding, Kristen M. ;
Bundy, Bradley C. .
BIOENGINEERED, 2017, 8 (04) :325-330