GOSSYPOL INHIBITION OF KIDNEY （Na++K+）-ATPase

被引：0
作者：
Fu Yun-feng
Lu Zhen-min
Wang Wei
机构：
[1] DepartmentofBiochemistry
[2] InstituteofExperimentalMedicine
[3] HebeiAcademyofMedicalSciences
[4] Shijiazhuang
来源：
CHINESE MEDICAL JOURNAL | 1987年 / 11期
关键词：
D O I：
暂无
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The inhibitory effect of gossypol acetic acid on the activity of (Na++K+)-ATPase partially purified from the outer medulla of rabbit kidney was examined, the IC50 is 6.5 μM. The enzyme kinetic studies show that gossypol is noncompetitive with ATP, Mg2+, Na+ and K+ with apparent Ki of 15, 13, 14.0 and 4.4 μM respectively. These data suggest that gossypol inhibition of (Na++K+)-ATPase is due to direct catalytic action at the subunit level, it may prevent proper positioning of the catalytic center or decrease the amount of active enzyme.
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页码：924 / 924
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