GOSSYPOL INHIBITION OF KIDNEY （Na++K+）-ATPase

被引：0

作者：

Fu Yun-feng

Lu Zhen-min

Wang Wei

机构：

[1] DepartmentofBiochemistry

[2] InstituteofExperimentalMedicine

[3] HebeiAcademyofMedicalSciences

[4] Shijiazhuang

来源：

CHINESE MEDICAL JOURNAL | 1987年 / 11期

关键词：

D O I：

暂无

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The inhibitory effect of gossypol acetic acid on the activity of (Na++K+)-ATPase partially purified from the outer medulla of rabbit kidney was examined, the IC50 is 6.5 μM. The enzyme kinetic studies show that gossypol is noncompetitive with ATP, Mg2+, Na+ and K+ with apparent Ki of 15, 13, 14.0 and 4.4 μM respectively. These data suggest that gossypol inhibition of (Na++K+)-ATPase is due to direct catalytic action at the subunit level, it may prevent proper positioning of the catalytic center or decrease the amount of active enzyme.

引用

页码：924 / 924

共 50 条

[11] KINETIC STUDIES ON INHIBITION OF (NA++K+)-ATPASE BY DIKETOCORIOLIN-B
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