V-ATPase,ScNhxlp and Yeast Vacuole Fusion

Membrane fusion is the last step in trafficking pathways during which membrane vesicles fuse with target organelles to deliver cargos. It is a central cellular reaction that plays important roles in signal transduction,protein sorting and subcellular compartmentation.Recent progress in understanding the roles of ion transporters in vacuole fusion in yeast is summarized in this article.It is becoming increasingly evident that the vacuolar proton pump V-ATPase and vacuolar Na~+/H~+ antiporter ScNhxlp are key components of the vacuole fusion machinery in yeast.Yeast ScNhxlp regulates vacuole fusion by controlling the luminal pH.V-ATPases serve a dual role in vacuolar integrity in which they regulate both vacuole fusion and fission reactions in yeast.Fission defects are epistatic to fusion defects.Vacuole fission depends on the proton translocation activity of the V-ATPase;by contrast,the fusion reaction does not need the transport activity but requires the physical presence of the proton pump.V,the membrane-integral sector of the V-ATPase,forms trans-complexes between the opposing vacuoles in the terminal phase of vacuole fusion where the Virons-complexes build a continuous proteolipid channel at the fusion site to mediate the bilayer fusion.