自噬诱导、抑制的人三阴性乳腺癌细胞系MDA-MB-231中ATG2A和ATG9A表达观察

被引：0
|
作者：
杨鑫娇 [1 ,2 ,3 ]
邹迪 [2 ,3 ]
熊喆 [2 ,3 ]
张海珠 [1 ]
夏从龙 [1 ]
赵红业 [1 ,2 ,3 ]
机构：
[1] 大理大学药学院
[2] 云南省动物基因编辑与体细胞克隆技术重点实验室
[3] 云南省异种器官移植工程研究中心
来源：
山东医药 | 2021年 / 61卷 / 21期
关键词：
乳腺肿瘤; 三阴性乳腺癌; ATG2A基因; ATG9A基因; 细胞自噬; MDA-MB-231细胞;
D O I：
暂无
中图分类号：
R737.9 [乳腺肿瘤];
学科分类号：
摘要：
目的观察自噬诱导、抑制的人三阴性乳腺癌细胞系MDA-MB-231中ATG2A、ATG9A表达变化。方法人三阴性乳腺癌细胞系MDA-MB-231分为4组,对照组加入DMEM培养液正常培养,Baf-A1组在DMEM培养液中加100 nmol/L的巴弗洛霉素A1(Baf-A1)进行自噬阻断,EBSS组加入Earle's平衡盐溶液(EBSS)进行饥饿诱导细胞自噬,EBSS+Baf-A1组在EBSS培养液中加入100 nmol/L的Baf-A1,四组均培养4 h。采用实时荧光定量(Q-PCR)法检测各组细胞中ATG2A、ATG9A mRNA,采用Western Blotting法检测各组细胞中ATG2A、ATG9A、P62、微管蛋白1轻链3(LC3)蛋白,计算LC3B-Ⅱ/LC3B-Ⅰ。结果与对照组、Baf-A1组相比,EBSS组、EBSS+Baf-A1组细胞中ATG2A、ATG9A mRNA相对表达量均显著升高(P均<0.05),且EBSS+Baf-A1组细胞中ATG9A mRNA相对表达量高于EBSS组(P均<0.05)。与对照组、Baf-A1组相比,EBSS组、EBSS+Baf-A1组细胞中ATG2A蛋白相对表达量均显著升高(P均<0.05);EBSS组细胞中ATG9A蛋白相对表达量均高于其余三组(P均<0.05);EBSS组细胞中P62蛋白相对表达量均低于其余三组(P均<0.05),EBSS+Baf-A1组P62蛋白相对表达量低于对照组(P均<0.05);EBSS组细胞中LC3B-Ⅱ/LC3B-Ⅰ均低于其余三组(P均<0.05),EBSS+Baf-A1组LC3B-Ⅱ/LC3B-Ⅰ均高于其余三组(P均<0.05)。结论 EBSS成功诱导MDA-MB-231细胞发生自噬,饥饿诱导的MDA-MB-231细胞自噬过程中ATG2A、ATG9A mRNA和蛋白表达升高。
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页码：13 / 17
页数：5
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