Degradation and biological performance of porous osteomimetic biphasic calcium phosphate in vitro and in vivo

The combination between biphasic calcium phosphate(BCP) and the osteomimetic porous microstructure obtained via freeze casting is hoped to achieve excellent bone regeneration, while the effects of HA and β-TCP ratio changes on the degradation and biological performance of the BCP scaffolds with this unique microstructure need to be determined. Here, we prepared the osteomimetic BCP scaffolds with different HA/β-TCP ratios(HA30/β-TCP70, HA50/β-TCP50, HA70/β-TCP30)and the effects of different HA/β-TCPHA/β-TCP ratios on the degradation and biological performance were studied in vitro and vivo. These BCP scaffolds with different HA/β-TCP ratios exhibited similar microstructure, mechanical performance, and protein absorption capability, while HA70/β-TCP30 BCP scaffolds showed an advisable degradation rate. Study in vitro confirmed the bio-compatibility and promotion on the proliferation, differentiation of MG63 cells in the porous osteomimetic BCP scaffolds with a HA/β-TCP ratio at 30:70. Implantation experiments also showed that the porous osteomimetic BCP scaffolds with a HA/β-TCP ratio at 30:70 had excellent bone regeneration capacity and proper degradation rate compatible with bone growth. These results reveal that the porous osteomimetic BCP scaffold with a HA/β-TCP ratio at 30:70 is a potential candidate of biodegradable bone substitutes used for bone repair.