Preliminary Study on in Vitro Induced Differentiation of Embryonic Stem Cells into Neurons

被引：3

作者：

Jian Ge Yan Guo Zhichong Wang Haichuan Liu Zhikuan Yang Yongping Li Shunong LiZhongshan Ophthalmic CenterPathophysiological DepartmentSun Yatsen University of Medical Sciences Guangzhou China ^{[510060
]}

机构：

来源：

眼科学报 | 2000年 / 01期

关键词：

embryonic stem cells; differentiation; neuron; glaucoma;

D O I：

暂无

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

<正> Purpose: To study preliminarily in vitro induced differentiation of embryonic stem cells into neurons for further investigation of an alternative for the treatment of glaumatous neuropathy.Materials and methods: Supernatant of cultured Buffalo rat liver cells (buffalorat liver cell-conditioned medium, BRL-CM) was used for culturing embryonic stem cells (ES-D3 cell line). Morphological features of undifferentiated ES cells were studied by HE staining and electron microscopy. Based on the methods used by Bain et al, we modified the methods and used retinoic acid (RA) as an inducer to differentiate ES-D3 cells and cytosine arabinoside (Ara-C) as inhibitor of proliferative cells. The growth of the cells was observed under phase contrast microscope.Results: ES-D3 cells cultured by BRL-CM grew in aggregates and remained undifferentiated. Electromicroscopy showed large nucleus and a large amount of mitochondria in undifferentiated ES cells and many processes on the surfaces. In the first day after the adding o

引用

页码：1 / 6

页数：6

共 10 条

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