Repairing articular cartilage defects with tissue-engineering cartilage in rabbits

被引:0
作者
宋红星 [1 ]
李佛保 [2 ]
沈惠良 [1 ]
廖威明 [2 ]
刘淼 [3 ]
王民 [3 ]
曹峻岭 [3 ]
机构
[1] Department of Orthopedics,Xuanwu Hospital,Capital Medical University,Beijing 100053,China
[2] Department of Orthopedics,First Affiliated Hospital,Sun Yat-Sen University,Guangzhou 510080,China
[3] Department of Orthopedics,First Affiliated Hospital,Xi'an Jiaotong University,Xi'an 710061,China
关键词
Cartilage; articular; Tissue engineering; Bone matrix; Gelatin;
D O I
暂无
中图分类号
R687.4 [关节手术];
学科分类号
1002 ; 100210 ;
摘要
Objective: To investigate the effect of cancellous bone matrix gelatin ( BMG ) engineered with allogeneic chondrocytes in repairing articular cartilage defects in rabbits. Methods: Chondrocytes were seeded onto three-dimensional cancellous BMG and cultured in vitro for 12 days to prepare BMG-chondrocyte complexes. Under anesthesia with 2.5% pentobarbital sodium (1ml/kg body weight), articular cartilage defects were made on the right knee joints of 38 healthy New Zealand white rabbits (regardless of sex, aged 4-5 months and weighing 2. 5-3 kg) and the defects were then treated with 2. 5% trypsin. Then BMG-chondrocyte complex ( Group A, n = 18 ), BMG (Group B, n = 10), and nothing (Group C, n = 10) were implanted into the cartilage defects, respectively. The repairing effects were assessed by macroscopic, histologic, transmission electron microscopic ( TEM ) observation, immunohistochemical examination and in situ hybridization detection, respectively, at 2, 4, 8, 12 and 24 weeks after operation. Results: Cancellous BMG was degraded within 8 weeks after operation. In Group A, lymphocyte infiltration was observed around the graft. At 24 weeks after operation, the cartilage defects were repaired by cartilage tissues and the articular cartilage and subchondral bone were soundly healed. Proteoglycan and type II collagen were detected in the matrix of the repaired tissues by Safranin-O staining and immunohistochemical staining, respectively. In situ hybridization proved gene expression of type II collagen in the cytoplasm of chondrocytes in the repaired tissues. TEM observation showed that chondrocytes and cartilage matrix in repaired tissues were almost same as those in the normal articular cartilage. In Group B, the defects were repaired by cartilage-fibrous tissues. In Group C, the defects were repaired only by fibrous tissues. Conclusions: Cancellous BMG can be regarded as the natural cell scaffolds for cartilage tissue engineering. Articular cartilage defects can be repaired by cancellous BMG engineered with allogeneic chondrocytes. The nature of repaired tissues is closest to the normal cartilage. Local administration of trypsin can promote the adherence of repaired tissues to host tissues. Transplantation of allogeneic chondrocytes has immunogenicity, but the immune reaction is weak.
引用
收藏
页码:266 / 271
页数:6
相关论文
共 11 条
[1]  
Yu J,Yan YH,Wan T.Progress of technology cartilage tissue-engineering. Orthop Biomech Mater & Clin Study . 2005
[2]  
Wu J,Sun JY.Progress of scaffold materials of cartilage tissue engineering. Orthopedic Clinics of North America . 2004
[3]  
Hu XB,Yao LL,Lu CZ, et al.Experimental and clinical investigations of human insoluble bone matrix gelatin A report of 24 cases. Clin Orthop Relat Res . 1993
[4]  
Buckwalter JA.Articular cartilage injuries. Clinical Orthopaedics and Related Research . 2002
[5]  
Chen KM,Liu XY,Ge BF, et al.Detection of type I and type II collagen mRNA in bone and cartilage by in situ hybridization. J Bone & Joint Injury . 2000
[6]  
Guo X,Lammi M,Aigner T, et al.Effect of low selenium on chondrocyte differentiation and differential expression of collagen types I, II and X in articular cartilage from mini-pigs. J Xi‘an Med Univ . 2000
[7]  
Zhang XH,Yu ZD,Tian XL, et al.Study on articular cartilage defect repaired with homograft of isolated and cultured chondrocytes. Chinese Journal of Traumatology . 1998
[8]  
Michael JA,Pasquale R,Daniel AG.Defining the challenge: The basic science of articular cartilage repair and response to injury. Sports Med & Arthroscopy Review . 2003
[9]  
Wei XQ,Cao JL,Xiong YM.The study on methods of rabbit chondrocytes culture. Chinese Journal of Endemiology . 1987
[10]  
Hunziker EB,Rosenberg LC.Repair of partial-thickness defects in articular cartilage: cell recruitment from the synovial membrane. Journal of Bone and Joint Surgery British Volume . 1996