Research on Quality Standards of Roots of Stemona sessilifolia( Miq. ) Miq.

[Objective]In order to control the quality of roots of Stemona sessilifolia(Miq.)Miq.,we established the standard of microscopic identification,thin-layer identification,moisture content,total ash,acid-insoluble ash,extractum and content determination.[Methods]Transverse section and powder were used in microscopic identification.Thin-layer identification of alkaloids was performed by TLC.Moisture content,total ash,acid-insoluble ash and extractum were determined by methods described in appendix of Chinese Pharmacopoeia.The content of protostemonine was measured by HPLC.Chromatography conditions.Phenomenex Gemini C6-phenyl.column(250 mm×4.6 mm,5μm);mobile phase:mix of acetonitrile and 0.1%triethylamine(34∶66,V/V);detection wavelength 306 nm;column temperature:30℃;flow rate:1.0 mL/min.[Results]Microscopic features of transverse section and powder were accordant with those recorded in Chinese Pharmacopoeia and documents.The number of phloem bundles and xylem bundles in stele differed.The chromatographic spot of thin-layer identification was very clear which indicated a good separation.Protostemonine displayed a good linear relation(R=0.999 8)when its concentration was6.257-156.42 mg/L.The average recovery rate reached 100.2%,(RSD 1.63%,n=6).[Conclusions]The method was simple to operate,accurate and had a good repeatability.It could be used to control the quality roots of Stemona sessilifolia(Miq.)Miq.