Analysis of SSRs Derived from an EST Library of Half-Smooth Tongue Sole Cynoglossus semilaevis

被引：0

作者：

LIU Yunguo BAO Baolong CHEN Songlin and SUN Xiuqin College of FisheriesOcean University of ChinaQingdao P R China Key Laboratory of Marine and Estuarine FisheriesMinistry of AgricultureEast China Sea Fisheries Research InstituteShanghai P R China College of AquaLife Science and TechnologyShanghai Ocean UniversityShanghai P R China Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao P R China The First Institute of OceanographyState Oceanic AdministrationQingdao P R China ^{[1
,2
,3
,4
,5
,1
,266003
,2
,200090
,3
,200090
,4
,266071
,5
,266061
]}

机构：

来源：

Journal of Ocean University of China | 2008年 / 03期

关键词：

half-smooth tongue sole; Cynoglossus semilaevis; EST; microsatellite;

D O I：

暂无

中图分类号：

S917.4 [水产动物学];

学科分类号：

0908 ;

摘要：

A complementary DNA(cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag(EST) database was generated,containing 3100 cDNA clones,of which 220 clones were fully sequenced. A total of 1060 non-redundant simple sequence repeats(SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats(51.4%) are the most abundant class of SSR in the dataset. The dimeric,trimeric,tetrameric and hexameric re-peats are represented in decreasing proportions of 27.2%,16.0%,2.8% and 1.9%,respectively. The frequency of pentameric repeats was observed the least(only 0.7%) . Most of the monomeric and dimeric repeats are distributed in 3' and 5' un-translation region. If translation regions are considered merely,trimeric repeats are the highest,accounting for 57% of the total microsatellites.

引用

页码：339 / 342

页数：4

共 4 条

[1] Simple sequence repeats as a source of quantitative genetic variation [J].

Kashi, Y ;

King, D ;

Soller, M .

TRENDS IN GENETICS, 1997, 13 (02) :74-78

[2] Polymorphism revealed by simple sequence repeats [J].

Powell, W ;

Machray, GC ;

Provan, J .

TRENDS IN PLANT SCIENCE, 1996, 1 (07) :215-222

[3]

Microsatellites, from molecules to populations and back[J] . Philippe Jarne,Pierre J.L. Lagoda.Trends in Ecology & Evolution . 1996 (10)

[4]

Pathological, Physiological, and Evolutionary Aspects of Short Unstable DNA Repeats in the Human Genome[J] . Patrik Künzler,Koichi Matsuo,Walter Schaffner.Biological Chemistry Hoppe-Seyler . 1995 (4)

← 1 →