In Vitro Developmental Potential of Cloned Embryos Derived from Bovine Somatic Cells and Rabbits Oocyte

reconstituted embryos were produced by nuclear transplantation using bovine ear fibroblasts at G 0 or non-G 0 stage as donor nuclei and oocytes collected from superovulated multiparous or young rabbits as recipients. After cultivation in two kinds of medium M199+10%FBS or RD+10%FBS, 112 of them developed to 2-cell stage (62.2%) and 26 to morula stage (14.4%) and 20 of them eventually developed to blastocyst stage (11.1%). There is no significant difference for the cleavage rates in two groups of reconstituted embryos derived from G 0 -stage and non-G 0 stage donor cells respectively. However, G 0-stage donor cells could result in higher rate of 8-cell16-cell stage embryos significantly (P<0.05), as well as higher rate of blastocysts (P<0.01). It seems that using two different culture systems had no significant effects on the cleavage rate, morula rate or blastocyst rate (P>0.05).