[Object] To analyze the essential oil chemical ingredients of Amomum tsao-ko from different regions of Yunnan Province,so as to provide references for its species identification and further utilization.[Method] Amomum tsao-ko samples were collected from Xiaoxin Village and Shangxincheng Village of Yuanyang,Lengquan Town of Mengzi,Damie Village of Wenshan,and the chemical ingredients of essential oil from Amomum tsao-ko leaves were identified by GC-MS.[Result] 61 chemical compounds were identified from 4 kinds of Amomum tsao-ko,among which the content of 9,12,15-gadusene-1-alcohol was the highest that exceeded 20%,eudesmol and palmitic acid took the second place which covered more than 10%.[Conclusion] There are abundant active ingredients in the essential oil of Amomum tsao-ko leaves,which deserves further research.