A rabies virus-based toolkit for efficient retrograde labeling and monosynaptic tracing

被引：0

作者：

KunZhang Lin ^{[1
,2
,3
]}

Lei Li ^{[3
]}

WenYu Ma ^{[3
,4
]}

Xin Yang ^{[3
]}

ZengPeng Han ^{[1
,2
,3
,4
]}

NengSong Luo ^{[1
,2
,3
,5
]}

Jie Wang ^{[3
,4
]}

FuQiang Xu ^{[1
,2
,3
,4
,5
,6
,7
]}

机构：

[1] The Brain Cognition and Brain Disease Institute (BCBDI),Shenzhen Key Laboratory of Viral Vectors for Biomedicine,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences

[2] Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Resea rch Institutions,NMPA Key Laboratory for Resea rch and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal Products,Shenzhen,Key Laboratory of Quality Control Tec

[3] State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,Kational Center for Magnetic Resonance in Wuhan,Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measu rement Science and Technology,Chinese Academy of S

[4] University of Chinese Academy of Sciences

[5] Wuhan National Laboratory for Optoelectro nics,Huazhong University of Science and Technology

[6] Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions

[7] Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences

来源：

NeuralRegenerationResearch | 2023年 / 18卷 / 08期

关键词：

D O I：

暂无

中图分类号：

R338 [神经生理学];

学科分类号：

0710 ; 071006 ;

摘要：

Analyzing the structure and function of the brain's neural network is critical for identifying the working principles of the brain and the mechanisms of brain diseases. Recombinant rabies viral vectors allow for the retrograde labeling of projection neurons and cell type-specific trans-monosynaptic tracing, making these vectors powerful candidates for the dissection of synaptic inputs. Although several attenuated rabies viral vectors have been developed, their application in studies of functional networks is hindered by the long preparation cycle and low yield of these vectors. To overcome these limitations, we developed an improved production system for the rapid rescue and preparation of a high-titer CVS-N2c-ΔG virus. Our results showed that the new CVS-N2c-ΔG-based toolkit performed remarkably:(1) N2cG-coated CVS-N2c-ΔG allowed for efficient retrograde access to projection neurons that were unaddressed by rAAV9-Retro, and the efficiency was six times higher than that of rAAV9-Retro;(2) the trans-monosynaptic efficiency of oG-mediated CVS-N2c-ΔG was 2–3 times higher than that of oG-mediated SAD-B19-ΔG;(3) CVS-N2c-ΔG could delivery modified genes for neural activity monitoring, and the time window during which this was maintained was 3 weeks; and(4) CVS-N2c-ΔG could express sufficient recombinases for efficient transgene recombination. These findings demonstrate that new CVS-N2c-ΔG-based toolkit may serve as a versatile tool for structural and functional studies of neural circuits.

引用

页码：1827 / 1833

页数：7

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