Establishment of Characteristic Chromatogram of Honeysuckle Stem Formula Granules Based on the Concept of Whole Process Quality Control

[Objectives] To establish a HPLC characteristic spectrum of honeysuckle stem formula granules, and to evaluate the quality consistency based on the standard decoction. [Methods] Using Agilent ZORBAX SB-C18(5 μm, 250 mm×4.6 mm) chromatographic column; gradient elution with acetonitrile-0.4% phosphoric acid solution(0.2:99.8, V/V), column temperature at 25℃; detection wavelength at 236 nm; flow rate 1.0 mL/min; using "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine", to evaluate the similarity of the characteristic chromatogram of crude drug, standard decoction and formula granules of honeysuckle stem. [Results] The similarity of characteristic chromatogram was higher than 0.94, and 6 components were identified from 10 characteristic peaks. [Conclusions] The established method can more comprehensively reflect the overall appearance of the chemical substances in the honeysuckle stem and the transmission law, and can provide an experimental basis for the consistency of the quality of the honeysuckle stem formula granules.