Curcumin-induced Histone Acetylation in Malignant Hematologic Cells

被引：0

作者：

胡俊斌 ^{[1
]}

王妍 ^{[2
]}

陈燕 ^{[1
]}

机构：

[1] Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

[2] Intensive Care Unit, Emergency Department, Nanjing Drum Tower Hospital

来源：

Journal of Huazhong University of Science and Technology(Medical Sciences) | 2009年 / 29卷 / 01期

关键词：

curcumin; histone acetylation; histone deacetylation;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

This study investigated the inhibitory effects of curcumin on proliferation of hemato-logical malignant cells in vitro and the anti-tumor mechanism at histone acetylation/histone deacety-lation levels. The effects of curcumin and histone deacetylase inhibitor trichostatin A (TSA) on the growth of Raji cells were tested by MTT assay. The expression of acetylated histone-3 (H3) in Raji, HL60 and K562 cells, and peripheral blood mononuclear cells (PBMCs) treated with curcumin or TSA was detected by immunohistochemistry and FACS. The results showed curcumin inhibited pro-liferation of Raji cells significantly in a time- and dose-dependent fashion, while exhibited low toxic-ity in PBMCs. Curcumin induced up-regulation of the expression of acetylated H3 dose-dependently in all malignant cell lines tested. In conclusion, curcumin inhibited proliferation of Raji cells selec-tively, enhanced the level of acetylated H3 in Raji, HL60, and K562 cells, which acted as a histone deacetylase inhibitor like TSA. Furthermore, up-regulation of H3 acetylation may play an important role in regulating the proliferation of Raji cells.

引用

页码：25 / 28

页数：4

共 3 条

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[2]

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[3]

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