Preliminary Validation of High Performance Liquid Chromatography Method for Detection of Methyl-testosterone Residue in Carp Muscle

被引：0

作者：

JIANG Jie LIN Hong FU Xiaoting and LI MingmingCollege of Food Science and Engineering Ocean University of China Qingdao PRChina ^{[266003
]}

机构：

来源：

Journal of Ocean University of China | 2005年 / 03期

关键词：

methyltestosterone; liquid-liquid extraction; HPLC; validation;

D O I：

暂无

中图分类号：

S917.4 [水产动物学];

学科分类号：

0908 ;

摘要：

The use of synthetic anabolic steroid methyltestosterone (MT) as growth promoter is prohibited in China. Vali-dations of analytical methods for MT residue in food and the results obtained have become indispensable. The high perfor-mance liquid chromatography (HPLC) method for the detection of MT with liquid-liquid extraction by trichloromethane-methanol in carp muscle tissue was preliminarily validated with reference to the following parameters: recovery (accuracy)at the 1, 5 and 10mg kg-1 level, between-run and within-run CV values (repeatability, also called relative standard deviation(RSD)) and limit of detection. The recoveries were above 80% and the between-run and within-run CV values below 10%for muscle tissue. The limit of detection was 0.05 mg kg-1.

引用

页码：60 / 63

页数：4