Germplasm Resources and Quality Research of Three Species of Chrysanthemums in Bozhou

[Objective] The paper was to preliminarily study the pharmacognosy of three species of chrysanthemums cultivated in Bozhou, and to compare their similarities and differences. [Method] Organs of three species of chrysanthemum samples were dissected and made into integral slices, and surface morphology of each part was observed microscopically. Powder slices were developed in the same way and observed under a mi-croscope. According to thin layer chromatography(TLC) test(Part I, Appendix VIB, 2005 edition), 2 μL of sample solutions and reference solutions were absorbed respectively, and dripped on the same piece of silica gel G thin layer plate. With petroleum ether-acetone(2∶1) as the developing solvent, samples were developed and then dried. Slices were sprayed with chromogenic agent 2% aluminum trichloride, and examined under UV light at the wavelength of 365 nm. Whether fluorescent spots of the same color were displayed in the position corresponding to the chromatogram of reference solutions were observed. [Result] Non-glandular hairs were found in bract microstructures of three species of chrysanthemums. There were many T-shaped hairs and few multicellular sessile non-glandular hairs. The epidermal cell walls of bracts were parallel straight, and some were rectangular; stomas could be seen everywhere. Only Dendranthema morifolium(Ramat.) Tzvel. ‘ Boju’ cv. nov. and Dendranthema morifolium(Ramat.) Tzvel. ‘Da maya’ cv. nov. displayed fluorescent spots of the same color in the position corresponding to the chromatogram of reference sample acacetin in TLC test, and the spots of D. morifolium(Ramat.) Tzvel.‘Da maya’ cv. nov. were light in color; there were no spots observed in Chrysanthemum morifolium Ramat. [Conclusion] The external characters, internal forms and internal components of D. morifolium(Ramat.) Tzvel.‘Boju’ cv. nov., D. morifolium(Ramat.) Tzvel. ‘Da maya’ cv. nov. and C. morifolium Ramat are all related to germplasm resources of chrysanthemum species. The contents of acacetin, one of the components of D. morifolium(Ramat.) Tzvel.‘ Boju’ cv. nov. and D. morifolium(Ramat.) Tzvel.‘Da maya’ cv. nov., remain to be further studied.