Durable superamphiphobic silica aerogel surfaces for the culture of 3D cellular spheroids

被引：0
作者：
Lianyi Xu [1 ]
Shuangshuang Chen [2 ]
Xuemin Lu [3 ]
Qinghua Lu [2 ,3 ]
机构：
[1] School of Materials Science, Institute of Energy Equipment Materials, Shanghai Dianji University
[2] School of Chemical Science and Engineering, Tongji University
[3] School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composite, Shanghai Jiao Tong University
来源：
National Science Review | 2019年 / 6卷 / 06期
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
cell spheroids; superamphiphobicity; durability; electrochemistry; silica aerogel;
D O I：
暂无
中图分类号：
O648.17 [凝胶及软胶];
学科分类号：
070304 ; 081704 ;
摘要：
The 3D multicellular spheroids with intact cell–cell junctions have major roles in biological research by virtue of their unique advantage of mimicking the cellular physiological environments. In this work, a durable superamphiphobic silica aerogel surface(SSAS) has been fabricated for the upward culture of 3D multicellular spheroids. Poly(3,4-ethylenedioxythiophene)(PEDOT) was first electrodeposited on a conductive steel mesh as a first template for porous silica coating. Soot particles were then applied as a second template to construct a cauliflower-like silica aerogel nanostructure. After fluorination, a hierarchical structure with re-entrant curvature was finally fabricated as a durable superamphiphobic surface. This superamphiphobic surface also presented excellent antifouling towards biomacromolecules and cells, which has been demonstrated by the successful upward culture of cell spheroids. The upward culture makes the observation of cellular behavior in situ possible, holding great potential for 3D cellular evaluation in vitro.
引用
收藏
页码：1255 / 1265
页数：11
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  • [1] Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture[J] . Khashayar Moshksayan,Navid Kashaninejad,Majid Ebrahimi Warkiani,John G. Lock,Hajar Moghadas,Bahar Firoozabadi,Mohammad Said Saidi,Nam-Trung Nguyen.Sensors ＆ Actuators: B. Chemical . 2018
  • [2] Design of spherically structured 3D in vitro tumor models -Advances and prospects[J] . L.P. Ferreira,V.M. Gaspar,J.F. Mano.Acta Biomaterialia . 2018
  • [3] Mass fabrication of uniform sized 3D tumor spheroid using high-throughput microfluidic system[J] . Bongseop Kwak,Yoohwan Lee,Jaehun Lee,Sungwon Lee,Jiseok Lim.Journal of Controlled Release . 2018
  • [4] Durable superhydrophobic and superamphiphobic polymeric surfaces and their applications: A review[J] . Kosmas Ellinas,Angeliki Tserepi,Evangelos Gogolides.Advances in Colloid and Interface Science . 2017
  • [5] Fabrication and characterization of low-cost, bead-free, durable and hydrophobic electrospun membrane for 3D cell culture[J] . Hajar Moghadas,Mohammad Said Saidi,Navid Kashaninejad,Amir Kiyoumarsioskouei,Nam-Trung Nguyen.Biomedical Microdevices . 2017 (4)
  • [6] Fabrication techniques for bioinspired, mechanically-durable, superliquiphobic surfaces for water, oil, and surfactant repellency[J] . Samuel Martin,Philip S. Brown,Bharat Bhushan.Advances in Colloid and Interface Science . 2017
  • [7] Electrochemically Tunable Cell Adsorption on a Transparent and Adhesion-Switchable Superhydrophobic Polythiophene Film
    Xu, Lianyi
    Chen, Shuangshuang
    Lu, Xuemin
    Lu, Qinghua
    [J]. MACROMOLECULAR RAPID COMMUNICATIONS, 2015, 36 (12) : 1205 - 1210
  • [8] Spheroform: Therapeutic Spheroid‐Forming Nanotextured Surfaces Inspired by Desert Beetle Physosterna cribripes[J] . Mihyun Lee,Kisuk Yang,Yong Hwa Hwang,Youngro Byun,Dong Yun Lee,Seung‐Woo Cho,Haeshin Lee.Adv. Healthcare Mater. . 2015 (4)
  • [9] Switchable Water‐Adhesive, Superhydrophobic Palladium‐Layered Silicon Nanowires Potentiate the Angiogenic Efficacy of Human Stem Cell Spheroids[J] . Jungmok Seo,Jung Seung Lee,Kihong Lee,Dayeong Kim,Kisuk Yang,Sera Shin,Chandreswar Mahata,Hwae Bong Jung,Wooyoung Lee,Seung‐Woo Cho,Taeyoon Lee.Adv. Mater. . 2014 (41)
  • [10] Microcavity substrates casted from self-assembled microsphere monolayers for spheroid cell culture
    Shen, Keyue
    Lee, Jungwoo
    Yarmush, Martin L.
    Parekkadan, Biju
    [J]. BIOMEDICAL MICRODEVICES, 2014, 16 (04) : 609 - 615
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