APCDD1 as a Co-receptor Positively Regulates Wnt5a/c-Jun Non-Canonical Signaling Pathway

被引：0

作者：

王磊 ^{[1
,2
]}

陶一昕 ^{[2
]}

张洁 ^{[2
]}

王晓晴 ^{[2
]}

周晌辉 ^{[3
]}

贺林 ^{[2
]}

马钢 ^{[1
,2
,4
]}

机构：

[1] Bio-X-Renji Hospital Research Center, Renji Hospital, School of Medicine, Shanghai Jiao Tong University

[2] Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University

[3] Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine

[4] Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University

来源：

Journal of Shanghai Jiaotong University(Science) | 2019年 / 24卷 / 04期

基金：

上海市自然科学基金; 中国国家自然科学基金; 国科技部“十一五”科技计划项目;

关键词：

adenomatosis polyposis down-regulated 1 (APCDD1); Wnt signaling; receptor; Wnt5a; polarized cell migration;

D O I：

暂无

中图分类号：

R363 [病理生理学]; TN911.7 [信号处理];

学科分类号：

0711 ; 080401 ; 080402 ; 100104 ;

摘要：

Adenomatosis polyposis down-regulated 1(APCDD1) is a transmembrane glycoprotein that negatively regulates Wnt/β-catenin canonical signaling by binding with Wnt ligands and receptors. We analyzed the role of APCDD1 in the Wnt5a/c-Jun non-canonical signaling pathway and demonstrated that APCDD1 can interact in vitro with Wnt5a, a classical ligand, and Ror2, a receptor of non-canonical Wnt signaling. Furthermore, we verified the binding of APCDD1 and Ror2 in primary cells of mouse skin. Moreover, APCDD1 seems to form a complex with Ror2 and Vangl2 in the cell, and complex formation can be improved by adding Wnt5a. In the presence of Wnt5a and Ror2, APCDD1 can induce the phosphorylation of c-Jun, a transcription factor of Wnt5a non-canonical signaling, and its phosphorylation level is a readout of Wnt5 a signaling. Wound-healing assay shows that APCDD1 accelerates polarized cell migration during Wnt5a-induced wound closure. Therefore,it is very likely that APCDD1 regulates Wnt5a/c-Jun non-canonical signaling as co-receptor binding with both Wnt5a and Ror2.

引用

页码：510 / 516

页数：7