Correlation Analysis of Quantitative Microscopic Characteristics and Polysaccharide Content of Dendrobium officinale from Guangxi

[Objectives] The paper was to investigate the correlation between the microscopic characteristic constants and the index component polysaccharide content of Dendrobium officinale from Guangxi. [Methods] The paraffin sections of D. officinale were made, and the microscopic characteristic constants of D. officinale stem, including cross section area, vascular bundle area and phloem area, were determined by NIS-Elements D microscopic image processing software. The contents of polysaccharides in D. officinale were determined by UV-Vis spectrophotometry, and the quantitative values of microscopic characteristics of D. officinale with different growth years were analyzed by SPSS 23.0(IBM SPSS Statistics 23.0) data statistical software. The correlation between quantitative microscopic characteristics of D. officinale and its polysaccharide content was analyzed. [Results] According to the correlation between the index component of D. officinale and its quantitative microscopic characteristics, the measurement indexes, the percentage of vascular bundle in the cross section area and the percentage of hard structure in the cross section area, were strongly negatively correlated with polysaccharide content. [Conclusions] The microscopic characteristic constant values of vascular bundles of D. officinale can be used as a method to verify polysaccharide content.