Protective effects of dl-3n-butylphthalide against diffuse brain injury

Dl-3n-butylphthalide can effectively treat cerebral ischemia;however,the mechanisms underlying the effects of dl-3n-butylphthalide on microcirculation disorders following diffuse brain injury remain unclear.In this study,models of diffuse brain injury were established in Sprague-Dawley rats with the vertical impact method.Dl-3n-butylphthalide at 80 and 160 mg/kg was given via intraperitoneal injection immediately after diffuse brain injury.Ultrastructural changes in the cerebral cortex were observed using electron microscopy.Cerebral blood flow was measured by laser Doppler flowmetry,vascular density was marked by tannic acid-ferric chloride staining,vascular permeability was estimated by the Evans blue method,brain water content was measured using the dry-wet method,and rat behavior was measured by motor function and sensory function tests.At 6,24,48,and 72hours after administration of dl-3n-butylphthalide,reduced cerebral ultrastructure damage,increased vascular density and cerebral blood flow,and improved motor and sensory functions were observed.Our findings demonstrate that dl-3n-butylphthalide may have protective effects against diffuse brain injury by ameliorating microcirculation disorder and reducing blood-brain barrier damage and cerebral edema.