黑果枸杞花青素结构差异对其稳定性及细胞抗氧化活性的影响

被引：5

作者：

邓楷 ^{[1
,2
]}

欧阳健 ^{[1
,2
,3
]}

胡娜 ^{[1
,3
]}

王洪伦 ^{[1
,3
]}

机构：

[1] 中国科学院藏药研究重点实验室

[2] 中国科学院大学

[3] 湖州中科高原生物创新中心

来源：

天然产物研究与开发 | 2022年 / 34卷 / 02期

基金：

浙江省自然科学基金;

关键词：

黑果枸杞; 花青素; 化学结构; 稳定性; 细胞抗氧化活性;

D O I：

10.16333/j.1001-6880.2022.2.005

中图分类号：

R285 [中药药理学];

学科分类号：

1008 ;

摘要：

本文研究了黑果枸杞花青素化合物结构对其稳定性和细胞抗氧化活性的影响。通过半制备型HPLC分离纯化得到了4种黑果枸杞花青素化合物并鉴定结构，利用比色法评价了化合物的稳定性、细胞毒实验以及H2O2诱导的SH-SY5Y细胞损伤模型评价了细胞抗氧化活性，并讨论了化学结构对其影响。结果表明反式结构比顺式结构在中性和弱碱性条件下有更好的稳定性，反式结构、糖基的增加以及母核B环上的甲氧基取代均可以通过缩短母核与糖链的分子距离从而提高稳定性，糖基的增加能显著地降低损伤细胞中的乳酸脱氢酶水平，且反式结构较顺式结构更有活性优势。综上所述，黑果枸杞花青素的结构影响稳定性和细胞抗氧化活性，但糖基的增加和酰基的反式异构对二者有更积极的意义。

引用

页码：213 / 219

页数：7

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