雷蒙德氏棉EST-SSRs分布特征及开发与利用

被引：22

作者：

王长彪

郭旺珍

蔡彩平

张天真

机构：

[1] 南京农业大学作物遗传与种质创新国家重点实验室

来源：

科学通报 | 2006年 / 03期

关键词：

雷蒙德氏棉; EST-SSRs; 分布; 分子标记; 多态性;

D O I：

暂无

中图分类号：

S562 [棉];

学科分类号：

0901 ;

摘要：

微卫星或简单重复序列(simplesequencerepeats,SSR)存在于表达序列标签(expressedsequencetags,ESTs)中.为了在棉花中开发EST-SSR功能性标记,利用生物信息学方法对NCBI网上公开的63485条雷蒙德氏棉(GossypiumraimondiiUlbrich)ESTs序列进行EST-SSRs特征分析.剔除冗余序列,得到非冗余序列58906条.在非冗余序列中发现含不同重复基元SSRs的EST序列有2620条,共2818个EST-SSRs,EST-SSRs序列的频率是4.45%,平均相隔14.8kb出现一个SSR.在1～6bp的重复基元中,三核苷酸重复基元的SSRs出现频率最高(38.31%),其次是二核苷酸(24.09%)、单核苷酸(23.35%).对所有的重复基元类型进行统计分析发现,所占比例最大的是A/T(18.67%),其次是AT/TA(14.83%).在复合型(compound)中发现三核苷酸串联三核苷酸的重复基元出现频率最高,为48.65%.利用Prime3软件,设计了1554对EST-SSRs引物,随机选用300对对本室四倍体作图亲本陆地棉TM-1和海岛棉海7124进行多态性检测,其中129对有多态性,多态性频率为43%.这些EST-SSRs将有效用于不同棉种间的分布特征比较及染色体定位等方面研究.

引用

页码：316 / 320

页数：5

共 14 条

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