Thirty Holstein Friesian cows with similar weight, age, calving number, lactating length, fat percentage, and same physiology condition were selected and randomly divided into 3 groups: group I, group II and group III, 20 g and 40 g Cysteamine /(cow·day) was supplemented into the normal diet for group I and group II, respectively; group III was used as control. Results showed that, group I and group II produced 4.86 % and 6.88 % more milk than that of control (P<0.05); Meanwhile, FCM (3.5 % fat) of group I and group II was increased by 4.98 % (P<0.01) and 6.56 % (P<0.01). Change of the milk fat among the three groups were not significant(P<0.05). Average daily milk protein yield significantly was increased by 4.14 % and 2.76 % respectively (P<0.05), though the milk protein content was slightly reduced. Moreover, somatic cells of the cow in the three groups were in normal physiological range in this trial.